產(chǎn)品屬性
Product Attributes
英文名稱
Malondialdehyde(MDA) Content Assay Kit
別名
丙二醛試劑盒 MDA Kit 丙二醛(MDA)試劑盒 丙二醛(MDA)試劑盒
檢測(cè)方法
微量法 Spectrophotometer/Microplate Reader
儲(chǔ)存條件
Store at 2-8℃,6 months
產(chǎn)品介紹
Product Introduction
氧自由基作用于脂質(zhì)的不飽和脂肪酸,生成過(guò)氧化脂質(zhì);后者逐漸分解為一系列復(fù)雜的化合物,其中包括丙二醛(MDA)。通過(guò)檢測(cè)MDA的水平即可檢測(cè)脂質(zhì)氧化的水平。
丙二醛(MDA)在酸性和高溫條件下,可以與硫代巴比妥酸(thiobarbituric acid,TBA)縮合,生成棕紅色的三甲川(3,5,5-三甲基惡唑-2,4-二酮),其最大吸收波長(zhǎng)在532nm。進(jìn)行比色后可估測(cè)樣本中MDA的含量。
注意事項(xiàng)
Considerations
1.本產(chǎn)品僅供科研使用。請(qǐng)勿用于醫(yī)藥、臨床診斷或治療,食品及化妝品等用途。請(qǐng)勿存放于普通住宅區(qū)。
2.為了您的安全和健康,請(qǐng)穿好實(shí)驗(yàn)服并佩戴一次性手套和口罩操作。
3.實(shí)驗(yàn)結(jié)果可由多種因素影響,相關(guān)處理只限于產(chǎn)品本身,不涉及其他賠償。
實(shí)驗(yàn)圖片
Experiment Image
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技術(shù)問(wèn)答
Technical Q&As
常見(jiàn)問(wèn)題
酶標(biāo)儀沒(méi)有相同的波長(zhǎng),可以用相近的嗎
2023-10-26
常見(jiàn)問(wèn)題
加熱后有紅色沉淀,上清偏黃正常嗎?有無(wú)解決辦法。<br /> 測(cè)出MDA值偏低怎么解決
2023-10-22
索萊寶助手
您好,請(qǐng)問(wèn)是什么加熱后有紅色沉淀呢?如果是MDA工作液,可能是沒(méi)有完全溶解好,可以劇烈振蕩或者超聲促融,完全混勻后看是否還是上清偏黃;如果MDA含量偏低,可以增加樣本量同時(shí)注意處理樣本是完全破碎樣本,使樣本中的MDA完全釋放出來(lái)。
常見(jiàn)問(wèn)題
細(xì)胞樣本如果沒(méi)有辦法用超聲處理應(yīng)該怎么辦
2023-07-20
索萊寶助手
您好,如果沒(méi)有超聲條件可以考慮勻漿或反復(fù)凍融去裂解提取。
常見(jiàn)問(wèn)題
檢測(cè)MDA必須檢測(cè)蛋白含量嗎
2023-06-23
索萊寶助手
您好,除了按蛋白濃度計(jì)算外還有其他計(jì)算公式您可以參考一下說(shuō)明書。
常見(jiàn)問(wèn)題
請(qǐng)問(wèn)在測(cè)定之前,樣品管顏色變化明顯嗎?除以細(xì)胞數(shù)量是細(xì)胞總數(shù)還是活細(xì)胞數(shù)呢
2023-06-12
索萊寶助手
您好,樣品測(cè)定管顏色變化與物質(zhì)含量有關(guān),用細(xì)胞數(shù)目計(jì)算指的是初始細(xì)胞總數(shù)。
常見(jiàn)問(wèn)題
請(qǐng)問(wèn)MDA GSH SOD的提取液都是一樣的么?可以共用么?
2023-02-20
索萊寶助手
您好,MDA、CAT、SOD、POD這幾個(gè)常用的盒子提取液是可以通用的,GSH則不是同一系列的了
常見(jiàn)問(wèn)題
貼壁細(xì)胞收集是用胰酶消化么 可以用細(xì)胞裂解液么<br />
2022-11-10
索萊寶助手
細(xì)胞收集可用胰酶消化,也可細(xì)胞刮刀處理。收集的細(xì)胞需用等滲緩沖鹽溶液洗滌1至2次。<br /> 提取細(xì)胞上清請(qǐng)參考試劑盒說(shuō)明書中具體條件,如需用裂解液處理,建議先做預(yù)實(shí)驗(yàn),依據(jù)具體結(jié)果進(jìn)行調(diào)整。
常見(jiàn)問(wèn)題
植物材料適用嗎?<br />
2022-09-15
索萊寶助手
可以檢測(cè)植物樣本,試劑盒沒(méi)有種屬限制。
常見(jiàn)問(wèn)題
和BC0020有什么區(qū)別
2022-08-29
索萊寶助手
和BC0020的反應(yīng)體系大小不一致,BC0020反應(yīng)體系比較大
常見(jiàn)問(wèn)題
蛋白濃度測(cè)定可以直接用加入了提取液的上清測(cè)定么
2022-08-28
索萊寶助手
提取液提取的上清可以檢測(cè)蛋白濃度
常見(jiàn)問(wèn)題
試劑三變黃色還可以用嗎
2022-08-03
索萊寶助手
您好,試劑三就是黃色的,是可以正常使用的
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